SLC47A1
Reactivity: Human, Rat, Mouse
WB, IF (cc)
Host: Rabbit
Polyclonal
AbBy Fluor® 750
Application Notes
Peptide ELISA: Antibody detection limit dilution 1/64,000. Western Blot: (0.1-0.3 μg/mL)A approx. 60 kDa band is observed in Human Kidney lysates. An additional band of unknownidentity was also consistently observed at 20 kDa. This band was successfully blocked byincubation with the immunizing peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
SLC47A1
(Solute Carrier Family 47, Member 1 (SLC47A1))
MATE1 antibody, 1300013J15Rik antibody, AI036982 antibody, mMATE1 antibody, zgc:113362 antibody, SLC47A1 antibody, DKFZp469B1821 antibody, RGD1311123 antibody, solute carrier family 47 member 1 antibody, solute carrier family 47, member 1 antibody, solute carrier family 47 (multidrug and toxin extrusion), member 1 antibody, MATE1 antibody, multidrug and toxin extrusion protein 2-like antibody, SLC47A1 antibody, Slc47a1 antibody, slc47a1 antibody, LOC100125995 antibody, LOC100220385 antibody
Background
Transporters are expressed in a wide variety of tissues where they perform the critical function of enabling anionic and cationic chemicals of exogenous and endogenous origin to cross otherwise impermeable cell membranes. The multidrug and toxin extrusion (MATE) transporters mediate cellular efflux of a variety of organic cations, including many drugs, and are involved in excretion of toxic electrolytes through urine and bile. Members of the MATE family share homology with the bacterial MATE protein family, which is responsible for drug resistance. MATE1 (multidrug and toxin extrusion protein 1), also known as SLC47A1, is a 570 amino acid multi-pass membrane protein that predominantly localizes to the plasma membrane but is also found in intracellular organelles. Expressed in adrenal gland, and to a lower extent in liver, skeletal muscle and kidney, MATE1 is responsible for the secretion of cationic drugs across the brush border membranes. MATE1 exists as three isoforms, which are produced by alternative splicing events.Synonyms: MATE-1, MATE1, Multidrug and toxin extrusion protein 1, Solute carrier family 47 member 1