This antibody is expected to recognize all five reported isoforms.
Cross-Reactivity (Details)
Species reactivity (expected):Bovine, Mouse, Rat. Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen
Peptide with sequence from the internal region of the protein sequence according to NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1. Genename: EWSR1
Peptide ELISA: 1/16000. Western Blot: 1 - 3 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
EWSR1 is a putative RNA binding protein of unknown function. Mutations in this gene, specifically at (11,22)(q24,q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in two products.Synonyms: EWS, EWS oncogene, EWSR-1, Ewing sarcoma breakpoint region 1 protein, RNA-binding protein EWS