GTPase NRas antibody (Internal Region)
-
- Target See all GTPase NRas (NRAS) Antibodies
- GTPase NRas (NRAS)
-
Binding Specificity
- Internal Region
-
Reactivity
- Human, Rat, Mouse, Dog
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This GTPase NRas antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Sequence
- C-RMKKLNSSDD GTQ
- Specificity
- This antibody detects NRAS.
- Cross-Reactivity (Details)
-
Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- Immunogen
- Synthetic peptide corresponding to internal sequence of Human NRAS (NP_002515.1). Genename: NRAS
- Top Product
- Discover our top product NRAS Primary Antibody
-
-
- Application Notes
-
Peptide ELISA: Antibody detection limit dilution 1/32000. Western Blot: 0.01-0.03 μg/mL. Approx.21 kDa band observed in Human lysates of cell lineA431. In transfected HEK293 transiently expressing NRAS bands of approx.21 kDa and21 kDa are observed. These bands are not observed in the non-transfected HEK293. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Small Intestine shows stainingof cytoplasm and membrane of enterocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- GTPase NRas (NRAS)
- Alternative Name
- NRAS (NRAS Products)
- Synonyms
- NRAS antibody, AV095280 antibody, N-ras antibody, ALPS4 antibody, NRAS1 antibody, NS6 antibody, N-RAS antibody, fj99a10 antibody, wu:fj99a10 antibody, zgc:100761 antibody, zras-b1 antibody, H-RAS antibody, HRAS antibody, GTPase NRas antibody, neuroblastoma RAS viral (v-ras) oncogene homolog L homeolog antibody, NRAS proto-oncogene, GTPase antibody, neuroblastoma ras oncogene antibody, neuroblastoma RAS viral oncogene homolog antibody, Tsp_01117 antibody, nras.L antibody, Nras antibody, NRAS antibody, nras antibody
- Background
- NRAS is an oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.Synonyms: ALPS4, GTPase NRas, HRAS1, N-Ras, Transforming protein N-Ras, neuroblastoma RAS viral (v-ras) oncogene homolog
- Molecular Weight
- 21 kDa (NP_002515.1).
- Gene ID
- 4893, 9606
- UniProt
- P01111
- Pathways
- p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling
-