DGAT1 antibody (Internal Region)

Catalog No. ABIN374862

Product Details anti-DGAT1 Antibody

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This DGAT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-QNSMKPFKDM DYS

Specificity: This antibody recognizes DGAT1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine (Dog).
Species reactivity (tested):Human, Rat.

Purification: Immunoaffinity Chromatography

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_036211.1.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western Blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DGAT1

Target: DGAT1 (Diacylglycerol O-Acyltransferase 1 (DGAT1))

Alternative Name: DGAT1 (DGAT1 Products)

Background: Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.Synonyms: AGRP1, CAT-related gene product 1, DGAT, DGAT-1, Diacylglycerol O-acyltransferase 1, Diglyceride acyltransferase

Gene ID: 8694

NCBI Accession: NP_036211

UniProt: O75907

Pathways: Hormone Transport