Neuroligin 2 antibody (Internal Region)
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- Target See all Neuroligin 2 (NLGN2) Antibodies
- Neuroligin 2 (NLGN2)
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Binding Specificity
- Internal Region
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Reactivity
- Human, Rat, Mouse, Dog
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Neuroligin 2 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- C-NPPDTDIRDP GKK
- Specificity
- This antibody detects Neuroligin-2.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse, Canine (Dog).
Species reactivity (tested):Human, Rat. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogen
- Peptide from the internal region of the protein sequence according to NP_065846.1 Genename: NLGN2
- Top Product
- Discover our top product NLGN2 Primary Antibody
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- Application Notes
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Peptide ELISA: antibody detection limit dilution 1: 8000. Western blot: Approx 90 kDa band observed in Human Pancreas lysates (calculated MW of90.0 kDa according to NP_065846.1). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 50 kDa. Thisband was successfully blocked by incubation with the immunising peptide. We wouldappreciate any feedback from people in the field - have any such results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Neuroligin 2 (NLGN2)
- Alternative Name
- Neuroligin-2 (NLGN2 Products)
- Synonyms
- Nlgn2 antibody, GB10066 antibody, NLGN2 antibody, CCE-L2 antibody, NV50031 antibody, Nv123771 antibody, NL2 antibody, RP23-422L16.24 antibody, neuroligin 2 antibody, LOC410779 antibody, NLGN2 antibody, LOC663865 antibody, LOC100123771 antibody, Nlgn2 antibody
- Background
- Neuroligins are cell adhesion proteins that are thought to instruct the formation and alignment of synaptic specializations. The three known rodent neuroligin isoforms share homologous extracellular acetylcholinesterase-like domains that bridge the synaptic cleft and bind beta-neurexins. All neuroligins have identical intracellular C-terminal motifs that bind to PDZ domains of various target proteins. Neuroligin 2 is exclusively localized to inhibitory synapses in rat brain and dissociated neurons. In immature neurons, neuroligin 2 is found at synapses and also at GABAA receptor aggregates that are not facing presynaptic termini.Synonyms: KIAA1366, NLGN2
- Gene ID
- 57555, 9606
- UniProt
- Q8NFZ4
- Pathways
- Cell-Cell Junction Organization, Synaptic Membrane
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