RAE1 antibody (C-Term)

Catalog No. ABIN374586

Product Details anti-RAE1 Antibody

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This RAE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-NAAEELKPRN KK

Specificity: This antibody recognizes RAE1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of Human RAE1 Genename: RAE1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/64000. Western Blot: 0.03-0.1 μg/mL. Detects a band of Approx 38-40 kDa in lysates of HumanHepatocarcinoma cell line HepG2.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RAE1

Target: RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))

Alternative Name: RAE1 (RAE1 Products)

Background: Mutations in Rae1 and Gle2 (Saccharomyces cerevisae homolog) genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Depletion of Rae1 from extracts or cells severely inhibits mitotic spindle assembly. Alternatively spliced transcript variants encoding the same protein have been found for this gene.Synonyms: MRNP41, mRNA export factor, mRNA-associated protein mrnp 41

Gene ID: 8480, 9606

UniProt: P78406

Pathways: SARS-CoV-2 Protein Interactome