NPM1 antibody (C-Term)

Catalog No. ABIN374536

Product Details anti-NPM1 Antibody

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Chimpanzee

Host: Goat

Clonality: Polyclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QEAIQDLWQW RKSL

Specificity: This antibody recognizes Nucleophosmin.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002511.1, NP_954654.1.

Application Details

Application Notes: Peptide ELISA: 1/2000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: Nucleophosmin (NPM1 Products)

Background: Nucleophosmin (NPM) also called B23, nutramin and NO38 is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. It is involved in the acute response of mammalian cells to environmental stress, such as UV rays. NPM continuously shuttles between the nucleus and cytoplasm. It has been shown to bind nucleic acid, prevent protein aggregation via its chaperone activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM is associated with the centrosome. It is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Because of NPM gene interaction with several tumor-associated chromosome translocations, NPM is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, it is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins.Synonyms: NPM, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Numatrin

Gene ID: 4869

NCBI Accession: NP_002511

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly