TPI1 antibody (C-Term)

Catalog No. ABIN374519

Product Details anti-TPI1 Antibody

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This TPI1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody is expected to recognise both reported isoforms of Triosephosphate isomerase (TPI1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-LKPEFVDIINAKQ, from the C Terminus of the protein sequence according to NP_000356.1, NP_001152759.1. Genename: TPI1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.01-0.03 μg/mL. Approx 26 kDa band observed in human liver and humanhepatoblastoma HepG2 cell lysates (calculated MW of 26.6 kDa according to NP_000356).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: Triosephosphate Isomerase (TPI1) (TPI1 Products)

Background: Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.Synonyms: TIM, TPI, Triose-phosphate isomerase

Gene ID: 7167, 9606

UniProt: P60174

Pathways: Cell RedoxHomeostasis