Pirin antibody (N-Term)

Catalog No. ABIN374451

Product Details anti-Pirin Antibody

Target: Pirin (PIR)

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Pirin antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GSSKKVTLSV LSREC

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the N Terminus of the protein sequence according to NP_003653.

Application Details

Application Notes: Peptide ELISA: 1/128,000. Western Blot: 0.3-1.0 μg/mL. Detects a band of Approx 30-35 kDa in Human Heart lysate. (Predicted Molecular Weight: 32.5 kDa).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Pirin

Target: Pirin (PIR)

Alternative Name: Pirin / PIR (PIR Products)

Background: The pirin gene encodes a member of the cupin superfamily. The protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor, as well as B cell lymphoma 3-encoded oncoprotein, suggest that the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication.Synonyms: 3-dioxygenase PIR, Probable quercetin 2, Probable quercetinase

Gene ID: 8544

NCBI Accession: NP_001018119

UniProt: O00625