ACTR1A antibody (C-Term)

Catalog No. ABIN374428

Product Details anti-ACTR1A Antibody

Target: ACTR1A (Alpha Centractin (ACTR1A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This ACTR1A antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-YEEDGARSIH RKT

Specificity: This antibody detects Centractin alpha at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, PigSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005727.1 Genename: ACTR1A

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 1000. Western blot: 1-3 μg/mL. Approx.45 kDa band observed in Human Kidney lysates(calculated MW of 42.5 kDa according to NP_005727.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ACTR1A

Target: ACTR1A (Alpha Centractin (ACTR1A))

Alternative Name: Centractin alpha (ACTR1A Products)

Background: The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50 % amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dynein mediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.Synonyms: ACTR1A, ARP1, Actin-RPV, CTRN1, Centrosome-associated actin homolog

Gene ID: 10121

NCBI Accession: NP_005727

UniProt: P61163

Pathways: M Phase