VPS28 antibody (C-Term)
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- Target See all VPS28 Antibodies
- VPS28 (Vacuolar Protein Sorting 28 (VPS28))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Dog, Pig
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This VPS28 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Sequence
- C-ESAYNAFNRF LHA
- Specificity
- This antibody detects VPS28 isoform 1 at C-term.
- Cross-Reactivity (Details)
- Species reactivity (expected):Mouse, Rat, Dog, PigSpecies reactivity (tested):Human
- Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- Immunogen
- Peptide from C Terminus of the protein sequence according to NP_057292.1 Genename: VPS28
- Top Product
- Discover our top product VPS28 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit dilution 1: 1000. Western blot: 1-3 μg/mL. Approx 24 kDa band observed in H460, Human Testis and Brainlysates (predicted MW of 24 kDa according to NP_057292.1). Immunohistochemistry: 10 μg/mL. In paraffin embedded Human Testis, stains acrosomalhead caps in round spermatids.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- Target
- VPS28 (Vacuolar Protein Sorting 28 (VPS28))
- Alternative Name
- VPS28 (VPS28 Products)
- Synonyms
- CG12770 antibody, DmVps28 antibody, Dmel\\CG12770 antibody, Dvps28 antibody, ESCRT-I antibody, ESCRT-II antibody, dVps28 antibody, dvps28 antibody, l(2)k16503 antibody, vps28 antibody, VPS28 antibody, GB12925 antibody, VPL13 antibody, VPT28 antibody, DDBDRAFT_0186436 antibody, DDBDRAFT_0234023 antibody, DDB_0186436 antibody, DDB_0234023 antibody, 1110014J03Rik antibody, AI847241 antibody, D730005C08Rik antibody, zgc:66078 antibody, Vacuolar protein sorting 28 antibody, VPS28, ESCRT-I subunit antibody, vacuolar protein sorting 28 homolog antibody, vacuolar protein sorting-associated protein 28 homolog antibody, ESCRT-I subunit protein VPS28 antibody, ESCRT I complex subunit Vps28 antibody, transport of soluble vacuolar hydrolase precursors antibody, subunit of the ESCRT-I complex antibody, vacuolar protein sorting-associated protein antibody, vacuolar protein sorting 28 family protein antibody, vacuolar protein sorting-associated protein 28 antibody, Vacuolar protein sorting-associated protein 28 homolog antibody, vacuolar protein sorting 28 antibody, vacuolar protein sorting 28 (yeast) antibody, vacuolar protein sorting 28 homolog S homeolog antibody, Vps28 antibody, VPS28 antibody, vps28 antibody, LOC408783 antibody, CpipJ_CPIJ003920 antibody, LACBIDRAFT_399656 antibody, PTRG_04781 antibody, MCYG_00028 antibody, PITG_04534 antibody, Tsp_08691 antibody, Tsp_08687 antibody, Tsp_08688 antibody, vps-28 antibody, vps28.S antibody
- Background
- Synonyms: ESCRT-I complex subunit VPS28, Vacuolar protein sorting-associated protein 28 homolog
- Gene ID
- 51160
- NCBI Accession
- NP_057292
- UniProt
- Q9UK41
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