EIF4ENIF1
Reactivity: Human, Mouse, Rat, Pig, Cow, Guinea Pig, Horse, Bat, Hamster, Monkey
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Peptide ELISA: 1/32000. Western blot: 0.25-0.5 μg/mL. Approx 140-150 kDa band observed in 293 lysates (predictedsize of approx.100 kDa according to NP_062817.1, however our observation agrees withthat of Dostie et al (see Ref.1). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
EIF4ENIF1 is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein mediates the nuclear import of eIF4E, by interacting with the importin alpha-beta complex. It is predominantly cytoplasmic, its own nuclear import is regulated by a nuclear localization signal and nuclear export signals.Synonyms: Eukaryotic translation initiation factor 4E nuclear import factor 1, Eukaryotic translation initiation factor 4E transporter, eIF4E transporter