ADAP1 antibody (C-Term)

Catalog No. ABIN374364

Product Details anti-ADAP1 Antibody

Target: ADAP1 (ArfGAP with Dual PH Domains 1 (ADAP1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Pig, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ADAP1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-QEYAVEAHFK HKP

Specificity: This antibody detects ADAP1 / CENTA1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Rat, Dog, PigSpecies reactivity (tested):Human, Mouse

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_006860.1 Genename: ADAP1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 40 kDa band observed in Human Brain and Mouse Brainlysates (calculated MW of 43.0 kDa according to NP_006860).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ADAP1

Target: ADAP1 (ArfGAP with Dual PH Domains 1 (ADAP1))

Alternative Name: ADAP1 / CENTA1 (ADAP1 Products)

Background: The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.Synonyms: Arf-GAP with dual PH domain-containing protein 1, Centaurin alpha 1, Centaurin-alpha-1, PM25, Putative MAPK-activating protein PM25

Gene ID: 11033

NCBI Accession: NP_006860

UniProt: O75689