FBXW2 antibody (C-Term)
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- Target See all FBXW2 Antibodies
- FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Cow, Dog, Pig
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This FBXW2 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Sequence
- C-KRGSSFLAGE HPG
- Specificity
- This antibody detects FBXW2 / FBW2 / FWD2 at C-term.
- Cross-Reactivity (Details)
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Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogen
- Synthetic peptide from C-Terminus of the protein sequence according to NP_036296.1 Genename: FBXW2
- Top Product
- Discover our top product FBXW2 Primary Antibody
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- Application Notes
- Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.3-1.5 μg/mL. Approx 48 kDa band observed in Human Kidney lysates(calculated MW of 48.8 kDa according to NP_036296.1). A stronger band of unknownidentity was also consistently observed at appr.38 kDa - both bands were successfullyblocked by incubation with the immunising peptide. Immunohistochemistry: 2-3 μg/mL. In paraffin embedded Human Kidney, showscytoplasmic staining of epithelial cells in some renal tubules.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FBXW2 (F-Box and WD Repeat Domain Containing 2 (FBXW2))
- Alternative Name
- FBXW2 / FBW2 / FWD2 (FBXW2 Products)
- Synonyms
- FBXW2 antibody, md6 antibody, fbw2 antibody, fwd2 antibody, MGC53244 antibody, FBW2 antibody, Fwd2 antibody, Md6 antibody, wu:fc72f10 antibody, zgc:110365 antibody, 2700071L08Rik antibody, MD6 antibody, F-box and WD repeat domain containing 2 antibody, F-box and WD repeat domain containing 2 L homeolog antibody, F-box and WD-40 domain protein 2 antibody, FBXW2 antibody, fbxw2 antibody, Fbxw2 antibody, fbxw2.L antibody
- Background
- F box proteins are an expanding family of eukaryotic proteins characterized by an approximately 40 amino acid motif, the F box. Some F box proteins have been shown to be critical for the ubiquitin mediated degradation of cellular regulatory proteins. In fact, F box proteins are one of the four subunits of ubiquitin protein ligases, called SCFs. SCF ligases bring ubiquitin conjugating enzymes to substrates that are specifically recruited by the different F box proteins. A large family of mammalian F box proteins has recently been identified and classified into three groups based on the presence of either the WD 40 repeats, the leucine rich repeats, or the presence or absence of other protein protein interacting domains. The FBXW2 gene product, the second identified member of the F box gene family, contains multiple WD 40 repeats.Synonyms: F-box and WD-40 domain-containing protein 2, F-box/WD repeat-containing protein 2, Protein MD6
- Gene ID
- 26190
- NCBI Accession
- NP_036296
- UniProt
- Q9UKT8
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