Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in lysates of cell lines HEPG2,HeLa and Jurkat (calculated MW of 46.0 kDa according to NP_003070.3). A 40 kDa band wasobserved in transfected HEK293 transiently expressing BAF57. This band is not observed inthe non-transfected HEK293. Full caption: A) HEK293 overexpressing BAF57 (RC209444) and probed with EABIN374349 (mocktransfection in first lane), tested by Origene. B) ABIN374349 staining (0.2 μg/mL) of Jurkat lysate (RIPA buffer, 35 μg total protein perlane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Target
SMARCE1
(SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily E, Member 1 (SMARCE1))