CARD6 antibody (N-Term)

Catalog No. ABIN374318

Product Details anti-CARD6 Antibody

Target: CARD6 (Caspase Recruitment Domain Family, Member 6 (CARD6))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CARD6 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: ATESTPSEII ERER-C

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_115976.2 Genename: CARD6

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: Approx.45-50 kDa and 25-30 kDa bands observed in A549, Human Heart andHuman Skeletal Muscle lysates at 1 μg of primary. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Pancreas shows variable

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for CARD6

Target: CARD6 (Caspase Recruitment Domain Family, Member 6 (CARD6))

Alternative Name: CARD6 (CARD6 Products)

Background: CARDs (caspase recruitment domain) are homotypic protein-protein interaction motifs belonging to the death domain (DD)-fold superfamily (reviewed in Werts et al, 2006). Homotypic interactions among CARD-containing proteins can occur with various proteins, including the inactive zymogen forms of caspases which have N-terminal pro-domains containing complementary CARDs. These interactions can result in the formation of multiprotein complexes, leading to protease cleavage and caspase activation. Most proteins containing a CARD are involved in signal transduction pathways that regulate apoptosis, adaptive immunity, or innate immunity including NF-kB activation and inflammation. There is a large number of CARD containing proteins, and the unique function of each remains to be fully elucidated. CARD6 is a CARD-containing protein with a domain structure that does not appear to be shared by any other protein (Stehlik et al, 2003). CARD6 has been found to associate with microtubules, and is structurally and potentially functionally related to the superfamily of interferon (INF)-inducible GTPases (Dufner, 2006). INF-inducible GTPases belong to a growing family of host defense proteins that confer cell immunity. Specifically, is thought that CARD6 may act as a pattern recognition receptor that combats pathogens in a cell-autonomous manner.Synonyms: Caspase recruitment domain-containing protein 6

Gene ID: 84674

NCBI Accession: NP_115976

UniProt: Q9BX69