ALMS1 antibody (C-Term)

Catalog No. ABIN374311

Product Details anti-ALMS1 Antibody

Target: ALMS1 (Alstrom Syndrome 1 (ALMS1))

Binding Specificity: C-Term

Reactivity: Human, Rat, Cow, Dog, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ALMS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-RVTNQLLGRK VPWD

Specificity: This antibody detects ALMS1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_055935.4 Genename: ALMS1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave bands at approx 200 kDa and 70 kDa in lysatesof Human T cell line Jurkat after 0.3 μg/mL antibody staining. Please note that currently wecannot find an explanation in the literature for the bands we observe given the calculatedsize of 460 kDa according to NP_055935.3. Both detected bands were successfully blockedby incubation with the immunizing peptide (and BLAST results with the immunizingpeptide sequence did not identify any other proteins to explain the additional bands). Wewould appreciate any feedback from people in the field - have any results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?Immunohistochemistry: 1-3 μg/mL. In paraffin embedded Human Testis shows staining ofcytoplasm in spermatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for ALMS1

Target: ALMS1 (Alstrom Syndrome 1 (ALMS1))

Alternative Name: ALMS1 (ALMS1 Products)

Background: ALMS1 contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined.Synonyms: Alstrom syndrome protein 1, KIAA0328

Gene ID: 7840

NCBI Accession: NP_055935

UniProt: Q8TCU4

Pathways: Sensory Perception of Sound, Carbohydrate Homeostasis, M Phase