UBE2L3 antibody (C-Term)

Catalog No. ABIN374293

Product Details anti-UBE2L3 Antibody

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2L3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-EFTKKYGEKR PVD

Specificity: This antibody detects UBE2L3 / UBCH7 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Cow.
Species reactivity (tested):Human, Mouse and Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_003338.1 Genename: UBE2L3

Application Details

Application Notes: Peptide ELISA: 1: 128000. Western blot: 0.5-2 μg/mL. Approx 18 kDa band observed in Hela and Jurkat lysates(predicted MW of 18 kDa according to NP_003338).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for UBE2L3

Target: UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))

Alternative Name: UBE2L3 / UBCH7 (UBE2L3 Products)

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). UBE2L3 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro.Synonyms: E2-F1, L-UBC, UBCE7, Ubiquitin carrier protein L3, Ubiquitin-conjugating enzyme E2 L3, Ubiquitin-protein ligase L3

Gene ID: 7332

NCBI Accession: NP_003338

UniProt: P68036