MRPL3
Reactivity: Human
Host: Rabbit
Polyclonal
FITC
Application Notes
Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 35 kDa band observed in lysates of cell line HepG2(calculated MW of 38.5 kDa according to NP_009139.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Target
MRPL3
(Mitochondrial Ribosomal Protein L3 (MRPL3))
Mammalian mitochondrial ribosomal proteins (MRPs) are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This antibody targets a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q.Synonyms: 39S ribosomal protein L3, L3mt, MRL3, MRP-L3, RPML3, mitochondrial, mitochondrial 60S ribosomal protein L3