Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-1 μg/mL. Approx 20-25 kDa band seen in human skeletal muscle lysate[Predicted MW of approx.25 kDa according to NP_055180]. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
CMT2L antibody, DHMN2 antibody, E2IG1 antibody, H11 antibody, HMN2 antibody, HMN2A antibody, HSP22 antibody, MGC64408 antibody, hsc70 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, fc09c11 antibody, wu:fc04b04 antibody, wu:fc09c11 antibody, zgc:64202 antibody, AU018630 antibody, AW413033 antibody, Cryac antibody, D5Ucla4 antibody, H11K antibody, HSP20-like antibody, Hsp22 antibody, heat shock protein family B (small) member 8 antibody, heat shock protein family B (small) member 8 L homeolog antibody, heat shock protein 8 antibody, heat shock protein b8 antibody, HSPB8 antibody, hspb8.L antibody, hspa8 antibody, hspb8 antibody, Hspb8 antibody
Background
Hsp22 (HSPB8) is a 196-amino acid protein that contains a central portion homologous to a highly conserved HSP-alpha crystallin domain common to all the small heat shock protein (HSP20) family members. Hsp22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. The highest abundance of Hsp22 is in skeletal muscle, heart, and placenta. Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L).Synonyms: Alpha-crystallin C chain, CRYAC, E2-induced gene 1 protein, E2IG1, Heat shock protein beta-8, Protein kinase H11, Small stress protein-like protein HSP22