Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (C-Term) antibody

Catalog No. ABIN374273

Product Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: C-RLIVAREHID KYKN

Specificity: This antibody detects ACADM at C-term. Expected to recognise both reported isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide from C-Terminus of Human ACADM protein (NP_000007.1, NP_001120800.1) Genename: ACADM

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial

Alternative Name: ACADM

Background: ACADM protein is a medium chain specific (C4 to C12 straight chain) acyl Coenzyme A dehydrogenase. The enzyme catalyzes the initial step of the mitochondrial fatty acid beta oxidation pathway. ACADM expression is induced during periods of fasting, when reliance on fatty acids for energy is increased. Clinical phenotypes are associated with ACADM hereditary deficiency.Synonyms: MCAD, Medium-chain specific acyl-CoA dehydrogenase mitochondrial

Gene ID: 34

NCBI Accession: NP_000007

UniProt: P11310