Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.5-2 μg/mL. Approx 22 kDa band observed in human kidney lysates(predicted MW of 22 kDa according to NP_006445). Please note the appearance of thisband was blocked by incubation with the immunising peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
MAD4 antibody, MST149 antibody, MSTP149 antibody, bHLHc12 antibody, xmad4 antibody, mst149 antibody, bhlhc12 antibody, mstp149 antibody, MGC84841 antibody, MXD4 antibody, 2810410A03Rik antibody, Mad4 antibody, MAX dimerization protein 4 antibody, Max dimerization protein 4 antibody, MAX dimerization protein 4 S homeolog antibody, microRNA 4800 antibody, MXD4 antibody, Mxd4 antibody, mxd4.S antibody, MIR4800 antibody, mxd4 antibody
Background
MAD4 is a member of the MAD gene family . The MAD genes encode basic helix loop helix leucine zipper proteins that heterodimerize with MAX protein, forming a transcriptional repression complex. The MAD proteins compete for MAX binding with MYC, which heterodimerizes with MAX forming a transcriptional activation complex. Studies in rodents suggest that the MAD genes are tumor suppressors and contribute to the regulation of cell growth in differentiating tissues.Synonyms: MAD-4, MAX dimerization protein 4, MXD4, Max-associated protein 4, Max-interacting transcriptional repressor MAD4