RPS27 antibody (N-Term)

Catalog No. ABIN374266

This Goat Polyclonal antibody specifically detects RPS27 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RPS27 antibody (N-Term) (ABIN374266)

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This RPS27 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RPS27 Antibody

Binding Specificity: N-Term

Sequence: PLAKDLLHPS PEE-C

Specificity: This antibody detects RPS27 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from N-Terminus of the protein sequence according to NP_001021.1 Genename: RPS27

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western Blot: 0.05-0.2 μg/mL. Approx 12 kDa band observed in lysates of T-cell line Jurkat(calculated MW of 9.2 kDa according to NP_001021.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RPS27

Target: RPS27 (Ribosomal Protein S27 (RPS27))

Alternative Name: RPS27

Background: Synonyms: 40S ribosomal protein S27, MPS-1, MPS1, Metallopan-stimulin 1

Gene ID: 6232

NCBI Accession: NP_001021

UniProt: P42677