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SF3B3 antibody (C-Term)

SF3B3 Reactivity: Human, Mouse, Rat, Cow, Dog WB Host: Goat Polyclonal unconjugated
Catalog No. ABIN374246
  • Target See all SF3B3 Antibodies
    SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))
    Binding Specificity
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 46
    • 30
    • 15
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse, Rat, Cow, Dog
    Host
    • 44
    • 2
    • 1
    Goat
    Clonality
    • 37
    • 10
    Polyclonal
    Conjugate
    • 28
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This SF3B3 antibody is un-conjugated
    Application
    • 27
    • 18
    • 10
    • 7
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Sequence
    C-VSKKLEDIRT RYAF
    Specificity
    This antibody detects SAP130 at C-term.
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human
    Purification
    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
    Immunogen
    Peptide from C Terminus of the protein sequence according to NP_036558.3 Genename: SF3B3
    Top Product
    Discover our top product SF3B3 Primary Antibody
  • Application Notes
    Peptide ELISA: Limit dilution 1: 2000. Western blot: 0.1-0.3 μg/mL. Approx 150 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 130 kDa according to NP_036558.3).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • Target
    SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))
    Alternative Name
    SF3B3 / SAP130 (SF3B3 Products)
    Background
    Synonyms: KIAA0017, Pre-mRNA-splicing factor SF3b 130 kDa subunit, SAP 130, SAP-130, SF3b130, STAF130, Spliceosome-associated protein 130, Splicing factor 3B subunit 3
    Gene ID
    23450
    NCBI Accession
    NP_036558
    UniProt
    Q15393
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