PAX8 antibody (N-Term)

Catalog No. ABIN374237

Product Details anti-PAX8 Antibody

Target: PAX8 (Paired Box 8 (PAX8))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Sequence: PHNSIRSGHG GLN-C

Specificity: This antibody is expected to recognise all five isoforms of Human PAX8.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_003457.1, NP_039245.1, NP_039246.1, NP_039247.1, NP_054698.1. Genename: PAX8

Application Details

Application Notes: Peptide ELISA: Antibody Detection Limit Dilution 1/32000. Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Thyroid Gland shows nuclear staining in epithelial cellsaround follicles. A customer found this antibody unsuccessful in Immunohistochemistry onMouse tissues.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

References for anti-PAX8 antibody (ABIN374237)

Borgogni, Sarchielli, Sottili, Santarlasci, Cosmi, Gelmini, Lombardi, Cantini, Perigli, Luconi, Vannelli, Annunziato, Adorini, Serio, Crescioli: "Elocalcitol inhibits inflammatory responses in human thyroid cells and T cells." in: Endocrinology, Vol. 149, Issue 7, pp. 3626-34, (2008) (PubMed).

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8 (PAX8 Products)

Background: This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells.Synonyms: Paired box protein Pax-8, Pax-8

Gene ID: 7849

NCBI Accession: NP_003457

UniProt: Q06710

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation