STUB1 antibody (C-Term)

Catalog No. ABIN374173

Product Details anti-STUB1 Antibody

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This STUB1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-DAFISENGWV EDY

Specificity: This antibody detetcs CHIP / STUB1 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_005852.2 Genename: STUB1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1 μg/mL. Approx 35 kDa band observed in Human Brain and SkeletalMuscle lysates (calculated MW of 34.5 kDa according to NP_005861.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for STUB1

Target: STUB1 (STIP1 Homology and U-Box Containing Protein 1 (STUB1))

Alternative Name: STUB1 / CHIP (STUB1 Products)

Background: Synonyms: CLL-associated antigen KW-8, Carboxy terminus of Hsp70-interacting protein, E3 ubiquitin-protein ligase CHIP, NY-CO-7, STIP1 homology and U box-containing protein 1

Gene ID: 10273

NCBI Accession: NP_005852

UniProt: Q9UNE7

Pathways: Regulation of Hormone Metabolic Process, Response to Water Deprivation