NUMB antibody (C-Term)

Catalog No. ABIN374158

Product Details anti-NUMB Antibody

Target: NUMB (Numb Homolog (NUMB))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This NUMB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequence: C-PFSSDLQKTF EIEL

Specificity: This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog) and Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity chromatography using the immunizing peptide

Immunogen: Synthetic peptide from the C-Terminus of Human NUMB (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1) Genename: NUMB

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.03-0.1 μg/mL. Approx 80 kDa band observed in Mouse Brain lysates(calculated MW of 70.0 kDa according to NP_001005743.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-NUMB antibody (ABIN374158)

Li, Fu, Zhang, Zhang, Xu, Ma, Li, Song: "The clathrin adaptor Numb regulates intestinal cholesterol absorption through dynamic interaction with NPC1L1." in: Nature medicine, Vol. 20, Issue 1, pp. 80-6, (2014) (PubMed).

Target Details for NUMB

Target: NUMB (Numb Homolog (NUMB))

Alternative Name: NUMB (NUMB Products)

Background: Protein NUMB homolog is an evolutionarily conserved protein that controls the differentiation of neuronal progenitor cells. Recent studies have revealed that NUMB functions in clathrin-dependent endocytosis by binding to the AP-2 complex. NUMB is also expressed in postmitotic neurons and plays a role in axonal growth. Neural cells expressing Numb isoforms with short phosphotyrosine-binding (SPTB) domain undergo extensive neurite outgrowth, an effect that can be blocked by voltage-gated Ca2+ channel (VGCC) inhibitor or by Ca2+ chelatorSynonyms: Protein S171, Protein numb homolog, h-Numb

Gene ID: 8650

NCBI Accession: NP_001005743

UniProt: P49757

Pathways: Cell-Cell Junction Organization