FOXP3 antibody (C-Term)
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- Target See all FOXP3 Antibodies
- FOXP3 (Forkhead Box P3 (FOXP3))
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Binding Specificity
- C-Term
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Reactivity
- Human, Pig, Dog
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This FOXP3 antibody is un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Sequence
- SQRPSRCSNP TPGP
- Specificity
- This antibody is expected to recognize both reported isoforms (NP_054728.2 and NP_001107849.1).
- Cross-Reactivity (Details)
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Species reactivity (expected):Canine (Dog) and Pig.
Species reactivity (tested):Human. - Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
- Immunogen
- Peptide from C-Terminus of the protein sequence according to NP_054728.2, NP_001107849.1. Genename: FOXP3
- Top Product
- Discover our top product FOXP3 Primary Antibody
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- Application Notes
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Peptide ELISA: Limit Dilution: 1/16000. Immunohistochemistry: 0.3-1 μg/mL. In paraffin embedded Human Tonsil, stains nuclei ofsome cells in the interfollicular area.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- FOXP3 (Forkhead Box P3 (FOXP3))
- Alternative Name
- FOXP3 (FOXP3 Products)
- Synonyms
- foxp3 antibody, FOXP3 antibody, AIID antibody, DIETER antibody, IPEX antibody, PIDX antibody, XPID antibody, JM2 antibody, scurfin antibody, sf antibody, RGD1562112 antibody, forkhead box P3 antibody, forkhead box P3a antibody, forkhead box P3 L homeolog antibody, FOXP3 antibody, foxp3a antibody, foxp3 antibody, foxp3.L antibody, Foxp3 antibody
- Background
- FOXP3 (a 431 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.Synonyms: Forkhead box protein P3, IPEX, JM2, Scurfin
- Gene ID
- 50943
- NCBI Accession
- NP_054728
- UniProt
- Q9BZS1
- Pathways
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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