MTA1 antibody (C-Term, Internal Region)

Catalog No. ABIN374108

Product Details anti-MTA1 Antibody

Target: MTA1 (Metastasis Associated 1 (MTA1))

Binding Specificity: C-Term, Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MTA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-PGDVFYMPKE

Specificity: This antibody detects MTA1 (Internal).

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near the C Terminus) of the protein sequence according to NP_004680.1 Genename: MTA1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.03-0.1 μg/mL. Approx 75 kDa band observed in Human Placenta lysates(calculated MW of 80.5 kDa according to NP_004680.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for MTA1

Target: MTA1 (Metastasis Associated 1 (MTA1))

Alternative Name: MTA1 (MTA1 Products)

Synonyms: Tex22 antibody, metastasis associated 1 antibody, metastasis associated 1 L homeolog antibody, MTA1 antibody, Mta1 antibody, mta1.L antibody

Background: MTA1 was identified in metastatic cells, specifically, mammary adenocarcinoma cell lines. Expression of this protein has been correlated with the metastatic potential of at least two types of carcinomas although it is also expressed in many normal tissues. The role it plays in metastasis is unclear. It was initially thought to be the 70 kDa component of a nucleosome remodeling deacetylase complex, NuRD, but it is more likely that this component is a different but very similar protein. These two proteins are so closely related, though, that they share the same types of domains. These domains include two DNA binding domains, a dimerization domain, and a domain commonly found in proteins that methylate DNA. The profile and activity of this protein suggests that it is involved in regulating transcription and that this may be accomplished by chromatin remodeling.Synonyms: Metastasis-associated protein MTA1

Gene ID: 9112

NCBI Accession: NP_004680

UniProt: Q13330

Pathways: Chromatin Binding