PRAM1 antibody (C-Term)

Catalog No. ABIN374107

Product Details anti-PRAM1 Antibody

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRAM1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DFCDPLENQP LPLGR

Specificity: This antibody detects PRAM1 at C-term.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_115528.3 Genename: PRAM1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Western blot: 1-3 μg/mL (overnight incubation). Approx 90-100 kDa band seen in Jurkat celllysates. Please note that although the predicted MW of PRAM-1 is 70 kDa, Moog-Lutz et al. ,(J. Biol. Chem, Jun 2001, 276: 22375 - 22381) detected aPRAM-1 migrating with an apparentmolecular mass of 97 kDa and this is what we observed as well.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumi

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for PRAM1

Target: PRAM1 (PML-RARA Regulated Adaptor Molecule 1 (PRAM1))

Alternative Name: PRAM1 (PRAM1 Products)

Background: Synonyms: PML-RARA-regulated adapter molecule 1, PRAM, PRAM-1

Gene ID: 84106

NCBI Accession: NP_115528

UniProt: Q96QH2

Pathways: Regulation of Leukocyte Mediated Immunity