SYN1 antibody

Catalog No. ABIN372716

Product Details anti-SYN1 Antibody

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Specific for the ~78k Synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse and Rat.

Purification: Affinity Chromatography.

Immunogen: Native protein purified from bovine brain.

Isotype: IgG

Application Details

Application Notes: Western blot: 1/1000. Immunoprecipitation: 1 μg per 200 μg lysate. Immunofluorescence: 1/2000 (Frozens Sections). Immunohistochemistry: 1/2000 (Frozens Sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 50 μL PBS (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Buffer: 5 mM Ammonium Bicarbonate.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted after reconstitution (in aliquots) at-20 °C.

References for anti-SYN1 antibody (ABIN372716)

Magupalli, Mochida, Yan, Jiang, Westenbroek, Nairn, Scheuer, Catterall: "Ca2+-independent activation of Ca2+/calmodulin-dependent protein kinase II bound to the C-terminal domain of CaV2.1 calcium channels." in: The Journal of biological chemistry, Vol. 288, Issue 7, pp. 4637-48, (2013) (PubMed).

Forman, Trojanowski, Lee: "TDP-43: a novel neurodegenerative proteinopathy." in: Current opinion in neurobiology, Vol. 17, Issue 5, pp. 548-55, (2008) (PubMed).

Tanabe, Shen, Liu, Campbell, Kuroha, Yamamoto, Engel: "The TR2 and TR4 orphan nuclear receptors repress Gata1 transcription." in: Genes & development, Vol. 21, Issue 21, pp. 2832-44, (2008) (PubMed).

Haycock, Kumer, Lewis, Vrana, Stockmeier: "A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope." in: Journal of neuroscience methods, Vol. 114, Issue 2, pp. 205-12, (2002) (PubMed).

Zhu, Klimek, Dilley, Haycock, Stockmeier, Overholser, Meltzer, Ordway: "Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression." in: Biological psychiatry, Vol. 46, Issue 9, pp. 1275-86, (1999) (PubMed).

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: Synapsin-1 (SYN1 Products)

Background: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989, Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002).Synonyms: Brain protein 4.1, SYN1, Synapsin I

Gene ID: 6853

NCBI Accession: NP_008881

UniProt: P17600