Dynamin 1 antibody (pSer774)
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- Target See all Dynamin 1 (DNM1) Antibodies
- Dynamin 1 (DNM1)
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Binding Specificity
- pSer774
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Reactivity
- Human, Mouse, Rat, Dog, Cow, Chicken, Non-Human Primate
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- This Dynamin 1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- Specific for the ~95k Dynamin protein phosphorylated at Ser774. This antiboy labels the purified protein phosphorylated in vitro by CDK5 but not by PKC. Does not cross react with other purified substrates of CDK5 (e.g. Amphiphysin and Synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.
- Cross-Reactivity (Details)
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Species reactivity (expected):Human, Bovine, Canine, Chicken, Mouse and non-Human Primates.
Species reactivity (tested):Rat. - Purification
- Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
- Immunogen
- Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser774 of rat Dynamin.
- Isotype
- IgG
- Top Product
- Discover our top product DNM1 Primary Antibody
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- Application Notes
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Western Blot: 1/1000. Immunohistochemistry (Fixed Cultured Neurons): 1/1000. (See Ref.3)Immunofluorescence (Fixed Cultured Neurons): 1/1000. (See Ref.3)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the antibody undiluted (in aliquots) at-20 °C.
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- Target
- Dynamin 1 (DNM1)
- Alternative Name
- Dynamin-1 (DNM1 Products)
- Synonyms
- DNM antibody, Dnm antibody, AI838169 antibody, Ftfl antibody, mKIAA4093 antibody, dnm antibody, DNM1 antibody, Dnm1 antibody, dnm1 antibody, si:dkey-246l19.2 antibody, dynamin 1 antibody, dynamin-1 antibody, dynamin 1a antibody, DNM1 antibody, Dnm1 antibody, BACOIKO008_37 antibody, PTRG_07818 antibody, PTRG_08097 antibody, dnm1 antibody, LOC100168088 antibody, LOC100199205 antibody, dnm1a antibody
- Background
- Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001, Graham et al., 2002, Tsuboi et al., 2002). Cyclin dependent protein kinase 5 phosphorylates Dynamin at Ser774 and Ser778 that are the phosphorylation sites on Dynamin phosphorylated in vivo (Tan et al., 2003). Phosphorylation of these sites on Dynamin is thought to play a key role in synaptic vesicle trafficking.Synonyms: DNM, DNM1
- Gene ID
- 140694
- NCBI Accession
- NP_542420
- UniProt
- P21575
- Pathways
- Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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