Collagen Type I antibody

Catalog No. ABIN372599

Product Details anti-Collagen Type I Antibody

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivity: Human, Mouse, Mammalian, Fish, Amphibian, Avian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Collagen Type I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Specific for the ~180 kDa Collagen I Alpha-1 Propeptide in Human lung fibroblast extract.

Cross-Reactivity (Details): Species reactivity (expected):most (if not all) Species of Mammals, Birds, Amphibians and Fishes.
Species reactivity (tested):Human and Mouse.

Purification: Affinity Chromatography.

Immunogen: Peptide from the Human Collagen I Alpha-1 propeptide sequence.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/1000. Immunohistochemitry: 1/100. The antibody also works well for Immunohistochemistry on Paraformaldehyde-FixedSections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in10 mM sodium citrate ( pH 6.0)/ 0.1 % Tween-20). Note that in Paraffin Sections of Formaldehyde-Fixed fibrotic mouse lung tissue, itrecognizes collagen I molecules that are still associated with the cells in which they weresynthesized.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS. Contains no preservative

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at-20 °C.

References for anti-Collagen Type I antibody (ABIN372599)

Marneros: "Effects of chronically increased VEGF-A on the aging heart." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 32, Issue 3, pp. 1550-1565, (2018) (PubMed).

Granata, Santoro, Masola, Tomei, Sallustio, Pontrelli, Accetturo, Antonucci, Carratù, Lupo, Zaza: "In Vitro Identification of New Transcriptomic and miRNomic Profiles Associated with Pulmonary Fibrosis Induced by High Doses Everolimus: Looking for New Pathogenetic Markers and Therapeutic Targets." in: International journal of molecular sciences, Vol. 19, Issue 4, (2018) (PubMed).

Goggins, Kakkad, Mironchik, Jacob, Wildes, Krishnamachary, Bhujwalla: "Hypoxia Inducible Factors Modify Collagen I Fibers in MDA-MB-231 Triple Negative Breast Cancer Xenografts." in: Neoplasia (New York, N.Y.), Vol. 20, Issue 2, pp. 131-139, (2018) (PubMed).

Target Details for Collagen Type I

Target: Collagen Type I (COL1) (Collagen, Type I (COL1))

Alternative Name: Collagen Type I (COL1 Products)

Synonyms: collagen type III alpha 1 chain antibody, COL3A1 antibody

Background: Collagen is the most abundant protein in mammals with type 1 collagen being the most abundant of the 29 known collagens. Type 1 collagen is integral in the strengthening and structural support of many tissues including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procoallagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).Synonyms: Alpha-1 type I collagen, Alpha-2 type I collagen, COL1A1, COL1A2

Gene ID: 1277

NCBI Accession: NP_000079

UniProt: P02452