Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificity
Recognizes Retinol Dehydrogenase 11 (all-trans/9-cis/11-cis) (RDH11). Cellular Localization: Endoplasmic reticulum membrane, Single-pass type II membrane protein.
Cross-Reactivity (Details)
Species reactivity (tested):Human.
Purification
Immunoaffinity Chromatography.
Immunogen
Synthetic peptide - KLH conjugated corresponding to N-terminal sequence MVELMFP (aa 1-7) and the C-terminal sequence LLGLPID (312-318) of the Human RDH11.
RDH11
Reactivity: Human
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin Sections: 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Buffer
PBS containing 0.02 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
RDH11 (Retinol dehydrogenase 11) exhibits an oxidoreductive catalytic activity towards retinoids. RDH11 is most efficient as an NADPH-dependent all-trans-retinal reductase. RDH11 is also involved in the metabolism of short-chain aldehydes. RDH11 is located in endoplasmic reticulum and is expressed at higher level in liver and testis and is expressed at lower levels in smooth muscle, thymus, submaxillary gland and epididymis. In testis, expression is restricted to pachytene spermatocytes. RDH11 is also expressed in four layers of the retina, including the outer segment of rods and cones. RDH11 belongs to the short-chain dehydrogenases/reductases (SDR) family.Synonyms: ARSDR1, CGI-82, HCBP12, HCV core-binding protein HCBP12, PSDR1, Prostate short-chain dehydrogenase/reductase 1, RalR1, Retinal Reductase 1, Retinol Dehydrogenase 11