DIABLO
Reactivity: Human
WB
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: 1/1,000-1/2,000. Western Blot: 1/5,000-1/10,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
100 mM Borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))
SMAC/DIABLO (second mitochondria-derived activator of caspase/direct IAP-binding protein with low PI) is a mitochondrial protein that functions as a regulatory component during apoptosis. It is encoded by a nuclear gene and is subsequently imported into the mitochondria where the N-terminus is removed generating the mature form of the molecule. SMAC/DIABLO has been shown to neutralize the antiapoptotic effect of the inhibitor of apoptosis protein (IAP) family, a major group of apoptosis regulators that have been shown to directly block caspase activity thereby blocking apoptosis. (Ref.1-6)Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase