This antibody is specific to ChT1 antigen (Mr 63/104 kDa) on Chicken thymocyte. (Ref.1) CT1 can block T cell differentiation in vitro, in thymic organ cultures and in thymocyte precursors cultured on stromal cell monolayers. (Ref.4) CT1 also recognizes quail cortical thymocytes. (Ref.1)
Characteristics
Synonyms: Cell surface A33 antigen, Glycoprotein A34, GPA34, V-set and immunoglobulindomain-containing protein 1
VSIG1
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Flow Cytometry: < / = 1 μg/10^6 cells. Identification and enumeration of ChT1+ cells. (Ref.4) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
PBS containing 0.09 % Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or in (aliquots) at -20 °C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
VSIG1
(V-Set and Immunoglobulin Domain Containing 1 (VSIG1))
ChT1, a member of the Ig superfamily with one V-like and one C2-like domain, is a T cell antigen that is expressed on the surface of embryonic thymocytes (day 10). In young chickens, about 90 % of the thymocytes as well as a subpopulation of peripheral lymphocytes (which represent recent thymic emigrants) are ChT1 positive. (Ref.1-4) Expression in the periphery declines with age and, in correlation with partial thymectomy, indicates that ChT1 can be used as an accurate marker for studying thymic function. (Ref.3)Synonyms: Cell surface A33 antigen, GPA34, Glycoprotein A34, V-set and immunoglobulin domain-containing protein 1