Adenosine A2a Receptor antibody (AA 373-391)

Catalog No. ABIN364458

Product Details anti-Adenosine A2a Receptor Antibody

Target: Adenosine A2a Receptor (ADORA2A)

Binding Specificity: AA 373-391

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenosine A2a Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Brand: IHC-plus™

Specificity: Detects adenosine receptor A2a. This antibody does not detect other AR subtypes. Species cross-reactivity: human. Not yet tested in other species.

Predicted Reactivity: Percent identity by BLAST analysis: Dog (100%) Gorilla, Marmoset, Panda (89%) Human, Horse (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide: ESHGDMGLPDVELLSHELK, corresponding to amino acids 373-391 of Dog Adenosine Receptor A2a. Percent identity by BLAST analysis: Dog (100%), Gorilla, Marmoset, Panda (89%), Human, Horse (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (1:50), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50. Western Blot: 1:1000 detects an ~45kD protein from canine striatum representing A2aAR. Immunohistochemistry (paraffin-embedded sections): 1:100. Staining of A2aAR in human hippocampus yields a pattern consistent with plasma membrane staining.

Comment:

Target Species of Antibody: Dog

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Adenosine A2a Receptor

Target: Adenosine A2a Receptor (ADORA2A)

Alternative Name: ADORA2A/Adenosine A2A Receptor (ADORA2A Products)

Synonyms: ADORA2A antibody, adora2a.1 antibody, zgc:194773 antibody, A2aR antibody, ADORA2 antibody, RDC8 antibody, A2AAR antibody, AA2AR antibody, A2ar antibody, ADENO antibody, Adora2l1 antibody, adora2a.2 antibody, zgc:193802 antibody, zgc:193822 antibody, adenosine A2a receptor antibody, adenosine A2a receptor a antibody, adenosine A2a receptor L homeolog antibody, adenosine A2a receptor b antibody, ADORA2A antibody, Adora2a antibody, adora2aa antibody, adora2a.L antibody, adora2a antibody, adora2ab antibody

Background: Name/Gene ID: ADORA2A
Subfamily: Adenosine
Family: GPCR

Synonyms: ADORA2A, Adenosine receptor A2a, ADORA2, A2a receptor, Adenosine A2a receptor, Adenosine A2 receptor, Adenosine receptor subtype A2a, HA2aR, RDC8

Gene ID: 135

UniProt: P29274

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, Synaptic Membrane, Feeding Behaviour, Cancer Immune Checkpoints