GHRL antibody (AA 103-117)

Catalog No. ABIN364403

Product Details anti-GHRL Antibody

Target: GHRL (Ghrelin and Obestatin Prepropeptide (GHRL))

Binding Specificity: AA 103-117

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This GHRL antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human GHRL / Ghrelin. This antibody is expected to recognise all reported isoforms.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Baboon, Monkey, Elephant (100%) Gorilla, Mouse, Hamster (93%) Marmoset, Rat, Pig (87%) Horse (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QDILWEEAKEAPADK from the C-terminus of human GHRL / Ghrelin (NP_057446.1, NP_001128413.1, NP_001128416.1, NP_001128417.1, NP_001128418.1). Percent identity by BLAST analysis: Human, Gibbon, Baboon, Monkey, Elephant (100%), Gorilla, Mouse, Hamster (93%), Marmoset, Rat, Pig (87%), Horse (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2 μg/mL), Peptide-ELISA (1:8000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GHRL

Target: GHRL (Ghrelin and Obestatin Prepropeptide (GHRL))

Alternative Name: GHRL / Ghrelin Preproprotein (GHRL Products)

Background: Name/Gene ID: GHRL

Synonyms: GHRL, Ghrelin, Growth hormone secretagogue, Motilin-related peptide, Obestatin, Appetite-regulating hormone, MTLRP, Protein M46

Gene ID: 51738

NCBI Accession: NP_057446, NP_001128413, NP_001128416, NP_001128417, NP_001128418

UniProt: Q9UBU3