14-3-3 theta antibody (AA 1-245)

Catalog No. ABIN364361

Product Details anti-14-3-3 theta Antibody

Target: 14-3-3 theta (YWHAQ)

Binding Specificity: AA 1-245

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This 14-3-3 theta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Purification: Protein G purified

Immunogen: Recombinant human 14-3-3 tau (1-245aa) purified from E. coli. NCBI Accession No.: NP_006817.

Type of Immunogen: Recombinant protein

Clone: AT1A1

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for 14-3-3 theta

Target: 14-3-3 theta (YWHAQ)

Alternative Name: YWHAQ / 14-3-3 Theta (YWHAQ Products)

Synonyms: 14-3-3 antibody, 14-3-3t antibody, 1c5 antibody, hs1 antibody, 1C5 antibody, HS1 antibody, 2700028P07Rik antibody, AA409740 antibody, AU021156 antibody, R74690 antibody, ywhaq antibody, ywhaq2 antibody, wu:fa91b01 antibody, wu:fb02e12 antibody, wu:fb36f06 antibody, wu:fk86h02 antibody, ywhaq1 antibody, ywhaz antibody, zgc:55499 antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta S homeolog antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a antibody, 14-3-3 protein theta antibody, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide b antibody, YWHAQ antibody, ywhaq antibody, Ywhaq antibody, ywhaq.S antibody, ywhaqa antibody, ywhaqb antibody

Background: Name/Gene ID: YWHAQ

Synonyms: YWHAQ, 14-3-3 protein T-cell, 14-3-3 protein theta, 14-3-3 protein tau, 14-3-3, 14-3-3 Tau, 1C5, Protein HS1, HS1, Protein tau

Gene ID: 10971

NCBI Accession: NP_006817

UniProt: P27348

Pathways: Apoptosis, Myometrial Relaxation and Contraction