Cathepsin L antibody (AA 114-333)

Catalog No. ABIN364356

Product Details anti-Cathepsin L Antibody

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Binding Specificity: AA 114-333

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cathepsin L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Purification: Protein G purified

Immunogen: Recombinant human Cathepsin L (114-333aa) purified from E. coli.

Type of Immunogen: Recombinant protein

Clone: 2H7

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000 - 1:2000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: CTSL / Cathepsin L (CTSL1 Products)

Background: Name/Gene ID: CTSL
Subfamily: Cysteine C1
Family: Protease

Synonyms: CTSL, Cathepsin L, Cathepsin L1, CATL, CTSL1, Major excreted protein, MEP

Gene ID: 1514

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades