TJP3 antibody (C-Term)

Catalog No. ABIN364296

Product Details anti-TJP3 Antibody

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Binding Specificity: C-Term

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TJP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Brand: IHC-plus™

Specificity: Reacts with ZO-3 protein with a molecular weight of ~130kD. Cross-reactivity with other Zonula Occluden family members has not been observed. Reactivity is confirmed with human T84 and MDCK cell lysates. Species cross-reactivity: human and canine.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide derived from the C-terminus of the human ZO-3 protein.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-P (10 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TJP3

Target: TJP3 (Tight Junction Protein 3 (Zona Occludens 3) (TJP3))

Alternative Name: TJP3 / ZO3 (TJP3 Products)

Synonyms: cb766 antibody, wu:fj93c08 antibody, zo-3 antibody, zo3 antibody, ZO-3 antibody, ZO3 antibody, tight junction protein 3 antibody, tight junction protein 3 S homeolog antibody, tjp3 antibody, tjp3.S antibody, TJP3 antibody, Tjp3 antibody

Background: Name/Gene ID: TJP3
Family: Occludin

Synonyms: TJP3, Tight junction protein 3, ZO-3, Zona occludens protein 3, Zonula occludens protein 3, Tight junction protein ZO-3, ZO3

Gene ID: 27134

UniProt: O95049