NDC80 antibody (AA 630-642)
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- Target See all NDC80 Antibodies
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
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Binding Specificity
- AA 630-642
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This NDC80 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NDC80 / HEC1.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (92%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-YEKKATLIKSSEE from the C-terminus of human NDC80 / HEC1 (NP_006092.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (92%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NDC80 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))
- Alternative Name
- HEC1 / NDC80 (NDC80 Products)
- Synonyms
- CG9938 antibody, Dmel\\CG9938 antibody, Hec1 antibody, NDC80 antibody, dNdc80 antibody, dmNdc80 antibody, hec1 antibody, KNTC2 antibody, ndc10 antibody, tid3 antibody, 2610020P18Rik antibody, AV139730 antibody, HEC antibody, HEC1 antibody, Kntc2 antibody, kntc2l antibody, wu:fb52e02 antibody, zgc:111801 antibody, HsHec1 antibody, TID3 antibody, hsNDC80 antibody, kntc2 antibody, NDC80, kinetochore complex component antibody, CG9938 gene product from transcript CG9938-RA antibody, spindle pole body protein Ndc80 antibody, kinetochore protein Ndc80 antibody, NDC80 homolog, kinetochore complex component (S. cerevisiae) antibody, NDC80 kinetochore complex component antibody, NDC80 kinetochore complex component S homeolog antibody, NDC80 antibody, Ndc80 antibody, ndc80 antibody, ndc80.S antibody
- Background
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Name/Gene ID: NDC80
Synonyms: NDC80, HEC1, Kinetochore protein Hec1, Kinetochore associated 2, HEC, HsHec1, HsNDC80, KNTC2, TID3 - Gene ID
- 10403
- NCBI Accession
- NP_006092
- UniProt
- O14777
- Pathways
- Maintenance of Protein Location
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