ERCC4 antibody

Catalog No. ABIN364262

Product Details anti-ERCC4 Antibody

Target: ERCC4 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4 (ERCC4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERCC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Recognizes XPF. Species cross-reactivity: human.

Purification: Protein G purified

Immunogen: Recombinant human XPF protein.

Type of Immunogen: Recombinant protein

Isotype: IgG2 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-2 μg/mL for 2hrs at RT. Positive control: MCF-7 cells or human tonsil.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERCC4

Target: ERCC4 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4 (ERCC4))

Alternative Name: ERCC4 / XPF (ERCC4 Products)

Background: Name/Gene ID: ERCC4

Synonyms: ERCC4, ERCC11, DNA repair endonuclease XPF, XPF

Gene ID: 2072

UniProt: Q92889

Pathways: DNA Damage Repair