PLP1 antibody (C-Term)

Catalog No. ABIN364261

Product Details anti-PLP1 Antibody

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Binding Specificity: C-Term

Reactivity: Human, Cow, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: Recognizes myelin proteolipid protein (PLP) in many mammalian species. This Recognizes DM20. PLP encodes the major protein components of compact CNS myelin and mutations in the PLP gene can lead to severe dysmyelinating disease.

Purification: Protein A purified

Immunogen: Synthetic peptide GRGTKF corresponding to C terminal region of myelin proteolipid protein.

Clone: Plpc1

Isotype: IgG2a

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide from tissue culture supernatant

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for PLP1

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Alternative Name: PLP1 / Myelin PLP (PLP1 Products)

Background: Name/Gene ID: PLP1

Synonyms: PLP1, HLD1, PMD, Myelin proteolipid protein, Proteolipid protein 1, SPG2, Lipophilin, MMPL, PLP, PLP/DM20

Gene ID: 5354