PP2A alpha/beta antibody

Catalog No. ABIN364225

Product Details anti-PP2A alpha/beta Antibody

Target: PP2A alpha/beta (PP2A A/B)

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Bat, Chicken, Cow, Dog, Drosophila melanogaster, Hamster, Horse, Insect, Monkey, Pig, Rabbit, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This PP2A alpha/beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human Protein Phosphatase 2 (Formerly 2A), Catalytic Subunit (PPP2CA/PPP2CB). Two variants NP_001009552.1 and NP_004147.1 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish, Seq squirt, Drosophila, Mosquito, Ant, Slime mold, Nematode (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EPHVTRRTPDYFL from the C-terminus of human Protein Phosphatase 2 (Formerly 2A), Catalytic Subunit (PPP2CA/PPP2CB) (NP_002706.1, NP_004147.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Catfish, Salmon, Smelt, Stickleback, Pufferfish, Zebrafish, Seq squirt, Drosophila, Mosquito, Ant, Slime mold, Nematode (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:32000), WB (0.05 - 0.2 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 35 kDa band observed in Human Brain (Cerebellum), Mouse Brain and Rat Brain lysates (calculated MW of 35.6 kDa according to NP_002706.1and NP_004147.1). Recommended concentration: 0.05-0.2 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PP2A alpha/beta

Target: PP2A alpha/beta (PP2A A/B)

Alternative Name: PP2A Alpha + Beta (PP2A A/B Products)

Background: This antibody detects: PP2A Alpha + Beta

NCBI Accession: NP_002706, NP_004147