APEX1 antibody

Catalog No. ABIN363379

Product Details anti-APEX1 Antibody

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Neutralization (Neut)

Specificity: Reacts with human ~37 kDa APE-1 protein

Cross-Reactivity: Mouse (Murine), Rabbit, Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with proteins from mouse, rat and rabbit.

Purification: Antigen affinity purified

Immunogen: Affinity purified human Apurinic/Apyrimidinic Endonuclease (APE/ref-1) fusion protein

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
- IHC: 1/1 000 - WB : 1/1 000 - B/N: 50-200 Molar excess - ChIP: 1/10-1/500 - ICC/IF: 1/50-1/200 - IP: 7 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Tris-glycine, NaCl 150 mM buffer, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: DNA-(Apurinic Or Apyrimidinic Site) Lyase (APE-1) (APEX1 Products)

Background: Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents.

Gene ID: 328

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus