ARNT antibody (AA 496-789)

Catalog No. ABIN363204

Product Details anti-ARNT Antibody

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Binding Specificity: AA 496-789

Reactivity: Human, Mouse, Rat, Cow, Sheep, Pig, Monkey, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This ARNT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS)

Specificity: Reacts with human HIF 1 beta /ARNT

Cross-Reactivity: Mouse (Murine), Cow (Bovine), Sheep (Ovine), Rat (Rattus)

Cross-Reactivity (Details): Cross reacts with proteins from bovine, sheep, mouse, rat and ferret. Not tested in other species.

Purification: Purified (protein G)

Immunogen: Fusion protein containing amino acids 496-789 of human HIF-1 beta

Clone: H1beta234

Isotype: IgG1 kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
ChIP1:10-1:500, IHC1:100, IHC-P 1:100, WB 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.4 mg/mL

Buffer: PBS, Sodium azide 0.05 %

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Target Details for ARNT

Target: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Alternative Name: Hypoxia-Inducible Factor 1-beta (ARNT Products)

Synonyms: HIF-1-beta antibody, HIF-1beta antibody, HIF1-beta antibody, HIF1B antibody, HIF1BETA antibody, TANGO antibody, bHLHe2 antibody, D3Ertd557e antibody, Drnt antibody, ESTM42 antibody, Hif1b antibody, W08714 antibody, mKIAA4051 antibody, Arnt1 antibody, ARNT antibody, zgc:136664 antibody, arnt1 antibody, hif1b antibody, tango antibody, xArnt antibody, hif1beta antibody, hif-1beta antibody, arnt.2 antibody, MGC68805 antibody, aryl hydrocarbon receptor nuclear translocator antibody, aryl hydrocarbon receptor nuclear translocator S homeolog antibody, ARNT antibody, Arnt antibody, arnt antibody, arnt.S antibody

Background: HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits which are bHLH proteins of PAS (PER, ARNT, SIM) family. HIF-1 beta forms heterodimer with AHR, AHRR, HIF1 alpha and EPAS1/HIF2 alpha as well as with other bHLH proteins and its dimerization is required for efficient DNA binding. Interact with TACC3 and NOCA7.

Gene ID: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effect