Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of total HDAC4/HDAC5/HDAC9 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen. Specificity/Sensitivity HDAC4/HDAC5/HDAC9 (Ab-246/259/220) Antibody detects endogenous levels of total HDAC4/HDAC5/HDAC9 protein.
Immunogen
Peptide sequence around AA 244-248, 257-261, 218-222 (T-A-S-E-P) derived from Human HDAC4, HDAC5, HDAC9. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.