Cofilin1/2 (CFL1/2) (AA 86-90) antibody

Catalog No. ABIN363074

Product Details

Target: Cofilin1/2 (CFL1/2)

Binding Specificity: AA 86-90

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Specificity: The antibody detects endogenous level of total cofilin1/cofilin2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 86-90 (A-T-Y-E-T) derived from Human coflin1, cofilin2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References

Li, Ke, Li, Liu, Zhu, Li: "DGCR6L, a novel PAK4 interaction protein, regulates PAK4-mediated migration of human gastric cancer cell via LIMK1." in: The international journal of biochemistry & cell biology, Vol. 42, Issue 1, pp. 70-9, (2009) (PubMed).

Target Details

Target: Cofilin1/2 (CFL1/2)

Alternative Name: cofilin1/cofilin2 (CFL1/2 Products)

Synonyms: CFL antibody, cofilin 1 antibody, CFL1 antibody

Background: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Molecular Weight: 19 kDa

NCBI Accession: NP_005498, NP_068733

UniProt: Q9Y281

Pathways: Tube Formation, CXCR4-mediated Signaling Events