Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of total IRS-1 protein.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.
Immunogen
Peptide sequence around AA 634-638 (P-M-S-P-K) derived from Human IRS-1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
IRS1
Reactivity: Human, Mouse, Rat
WB, IHC, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Ekuni, Tomofuji, Irie, Kasuyama, Umakoshi, Azuma, Tamaki, Sanbe, Endo, Yamamoto, Nishida, Morita: "Effects of periodontitis on aortic insulin resistance in an obese rat model." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 90, Issue 3, pp. 348-59, (2010) (PubMed).
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit